Bioimaging-GR Infrastructure is an initiative aiming at the creation of a state-of-the-art network of infrastructure for biological visualisation in Greece, within the framework of Euro-BioImaging, which is the relevant infrastructure of the pan-European ESFRI research infrastructure map (http://www.eurobioimaging.eu/). Bioimaging methodologies are now widely used and with increasing intensity and acceptance as cutting-edge tools to study complex biological phenomena, understand cell structure and function, and extract biological information related to clinical practice. Recent developments in imaging technologies and the development of various innovative microscopy techniques have opened up new horizons in the field of Biological Imaging, which quickly becomes critical in the area of Health Sciences. The BioImaging-GR project aims to create a strongly interconnected and geographically distributed infrastructure for general access to advanced biological imaging techniques, which provide a variety of imaging methods to scientists, stakeholders, small and medium-sized enterprises (SMEs) and companies in Greece and neighbouring countries. In addition, BioImaging-GR will seek to acquire and provide access to imaging technologies beyond the level of knowledge, which are not easily accessible to the wider imaging community. In addition to the acquisition and upgrading of the facilities, BioImaging-GR will introduce new skills related to biomedical decontamination through the recruitment and training of permanent staff. Through BioImaging-GR, national investment in imaging infrastructure will be used in the most efficient and cost-effective way, applying the highest quality standards in the management, access and service of imaging facilities. The modernisation of bioimaging infrastructure and the acquisition or creation of the necessary know-how will allow Greece to maintain its competitive position in biomedical research, and to provide cutting-edge facilities to the wider research community in south-eastern Europe.