Confocal microscopy is a basic technology for cellular biology research that improves the resolution in fluorescent fields, mainly in depth (Z), allowing exact 3D reconstructions, with a reduction in phototoxidity that leads to an optimal videomicroscopy of live cells. A basic equipment of laser confocal microscopy is requested in this project; its set up has been defined to use microscopy of fluorescent proteins (blue, green, yellow and red) in live cells, as well as DNA staining with DAPI and immunofluorescence using the most widely used dyes (ie. Alexa Fluor group), including the most recent within the infrared spectrum. Therefore, the requested equipment includes a basic inverted microscopy with laser scanning head, five lasers (405, 488, 512, 552 y 638 nm) and three detectors, two of them of high sensitivity, specifically designed for fluorescent proteins. The main uses of this microscope include: (i) multicolor videomicroscopy of yeasts and live cells from mammals (CFP, GFP, YFP, RFP), including experiments IRIF, FRAP y FLIP; and (ii) cell and tissue immunofluorescence, including primary cell cultures. This confocal microscope will be placed in a specific space dedicated to microscopy at the Research Unit of the Hospital Universitario Nuestra Señora de Candelaria.