The gall of the collar, a disease of the vine caused by Agrobacterium vitis, results in a decrease in yield of about 20-40 % and sometimes the death of the plant. In nature, various biotic and abiotic (cold) constraints influence the process of A. vitis-mediated infection. A. vitis is present throughout the vineyard in the latent state until it finds favourable conditions for its development (following heavy winter frost or hail causing damage or bursting). Tumors then form and destroy the vascular system, resulting in the death of the young vine strain. Since the pathogen is systemic, there is no effective means of chemical control to date. A bacterial strain Paraburkholderia phytofirmans PsJN capable of (i) protecting the vine against numerous biotic and biotic stress, (ii) promote the growth of the vine (roots and aerial parts) after inoculation via the root system has been studied for many years in the RIBP laboratory. PsJN has the same ecological niche (xylem vessels) as A. vitis and protects the vine against frost, the main cause of the spread of A. vitis. We have shown that the PsJN strain induces a significant reduction in tumour size. The objective is to optimise the use of the PsJN bacterial strain or PsJN synthesised elcitor biomolecules as a new means of biocontrol to control the gall of collar in vines.