The main objective of the project will be the development and application of genetically encoded biosensors and the development of containment processes for natural compounds with high added market value, creating high efficiency analytical tools to accelerate the development of existing and new biorefinery schemes and improve biological production processes. Natural products such as essential amino acids, phenolic compounds, milk and other organic acids have great potential for agricultural innovation and food technologies. In recent years, efforts have been made to develop more sustainable processes for the production and extraction of these compounds. Currently, the quantification of organic acids depends very much on time-consuming procedures that require expensive chromatography equipment. Spectrophotometric methods suffer from poor selectivity indicators and provide inaccurate evaluation results. In addition, the engineering of microorganisms for the production of metabolites requires rapid and efficient methods for selecting large strain libraries. Therefore, high-performance technologies are needed to quickly and accurately identify the high added value of these products. Due to their high sensitivity and specificity to ligands (natural products), it is effective to carry out rapid analysis of many analytical samples,genetically encoded biosensor is a promising sanalising tool. KTU Food Science and Technology Department focuses on the processing of plant material and their by-products in order to optimise extraction technologies and identify added market value natural products. Based on this activity, the main goal of the project will be to create a set of biosensors and to apply as an analytical tool for the detection and quantification of natural compounds. The project will provide new tools and technologies for both scientific and practical applications to facilitate the development of new biorefining methodologies.