In the European Union and Lithuania, it is planned to support the production of second and next generation biofuels (from waste and non-food feedstocks) by identifying a 60 % reduction in CO2 emissions over the life cycle, addressing ecological and energy problems. The development of sustainable transport and energy requires the development of liquid and gaseous biofuels and the development of technologies for the use of these alternative fuels. The aim of the project is to experiment with factors influencing the ecological performance of a compression ignition engine operating on alternative biofuels. As part of the project “Study on the feasibility of reducing emissions from automotive transport by replacing fossil fuels with alternative fuels produced from bio-waste” the student will acquire knowledge and practical experience in the field of research on the performance of internal combustion engines. The student will master the research methodology, familiarise himself with the principles of analysis of the results obtained, examining the ecological and energy indicators of internal combustion engines, and evaluate the positive aspects of the use of alternative biofuels. The results of the study would allow a wider use of biofuels (biodiesel and biogas) in transport and energy by developing the production of alternative fuels using bio-waste, thus reducing environmental pollution than greenhouse gas emissions.