Terpenes are compounds of isoprene origin, both in flora and fauna and in microorganisms, which, thanks to more than 55 000 known molecules, constitute the largest class of natural substances. In addition to a high degree of structural diversity, their pharmacological properties are very diverse, many of their representatives are on the market as medicines or a promising lead molecule in drug research. Nearly two thirds of the new medicines placed on the market over the last two and a half decades were developed on the basis of a plant, animal or microbial substance (1). Developments based on new herbal and microbial compounds prove the rationale of drug research focused on natural substances in the era of modern pharmaceutical research technologies (combinatorical chemistry, HTS filter systems, bioinformatics, proteomics, genomics). At the same time, drug research based on natural substances has a number of difficulties, disadvantages e.g. low productivity, excessive complexity of molecules (production difficulties), problems with repeatability and difficult handling in screening systems. It is now clear that natural active substance research can be effective by integrating modern methods of drug research (HTS, CC) (2,3). Today, metabolomics and metagenomic strategies have been established in the world’s leading natural matter research sites, and HTPS incompatibility is solved by appropriate prefraction techniques (4). The technical development of large instruments helps to minimise the greatest obstacle to natural-based drug research, the time factor needed to isolate compounds. Metabolomic research, which takes a few years and is considered to be extremely promising for drug research internationally, makes it possible to identify the compounds responsible for the effect without the need to isolate them, which greatly shortens the process of identifying guide molecules. This speed gives priority to those active in the field, which is also of paramount importance for scientific disclosure and exploitation in the (pharmaceutical) industry. In the framework of this project, we apply a system metabolomic approach to the search for potential drug candidates, the instrumental background of which is a metabolomic (LC-MSn-based) profile study with a highly permeable in vitro pharmacological screening test, and with the help of which we seek correlations between bioactivity and changes in the chemical composition of naturally sourced extracts. This method allows for the rapid identification of active substances by bypassing the steps of isolation of the active substance previously considered to be speed-determining. The systemic approach also applies to the selection of species selected for testing: the study of taxa related to species that appear to be perspective based on ethno-pharmacological knowledge serves to improve the hit rate. The metabolomic studies accompanied by HTS pharmacological testing, and the detailed pharmacological-toxicological and molecular optimisation researches, result in the creation of an internationally unique research potential and a knowledge centre and a compound bank. The objective of this project is to create a research workshop that aims to identify drug-labelled molecules with a system metabolomic approach and high efficiency in the field of natural substances. The primary purpose of chemical and pharmacological studies is to identify and semi-synthetically optimised compounds with antitumor and microbial action (and to enhance the efficacy of these therapies) and cardiovascular activity in terms of the effect to be achieved. The planned work is based on research on disease groups that can be relevant for the health of populations in both developing and developed countries, integrating international best practices. The use of bioactive compounds identified in the research in human medicine requires the development of innovative pharmaceutical collaborations, so promising results can generate company collaborations and strengthen the development of the knowledge-based economy. This will also lead to a higher level of education for doctoral students in research teams and graduate students trained by participating workshops. B) The purpose of this project is to investigate and investigate biologically active guide molecules of natural origin, derived from plants, moss, large mushrooms and microbes (bacteria, fungi). The focus of our work is on antitumor, anti-infective and cardiovascular-acting compounds, as according to literature data, research on natural active substances mainly results in drug-labelled molecules in these areas and, on the other hand, tumour, cardiovascular and infectious diseases are the basis for the indicator of mortality and public health in developed and developing countries