A) In the last decade, the University of Pannonia has become increasingly prominent and has achieved internationally recognised results in the development of bio-nanotechnology research, especially in the development of new diagnostic methods and the creation of self-organised nanostructures based on biomolecules. The established biomolecular nanotechnology knowledge base was further strengthened by intensive cooperation with the Institute of Materials and Environmental Chemistry (AKI) of the Hungarian Academy of Sciences in the field of the creation, characterisation and application of structural and functional nanoparticles. In order to further strengthen scientific excellence in bio-nanotechnology, in conjunction with the priorities of the National Smart Specialisation Strategy and key smart technologies, the University of Pannonia intends to become an internationally recognised institution in the development of nanodiagnostic processes, research and production of smart nanomaterials. In order to achieve this objective, we submit a proposal involving three sub-projects with the following focal points based on existing research experience: (1) the development of micro- and nanodiagnostic procedures for effective disease detection, (2) the creation of functional nanoparticles in medicine and environmental analytics, and (3) the production of self-organised intelligent nanomaterials. Successful implementation of the project requires a multidisciplinary approach. Prof. András Guttman, external member of the Hungarian Academy of Sciences, who has repeatedly demonstrated his expertise and project leadership skills in the field of research (Lendület III. #97101, Marie Curie Chair #006733, Fulbright #48421907). The project is in line with international trends, thus helping to increase the international competitiveness of Hungarian bio-nanotechnology research. By further strengthening the existing state-of-the-art infrastructural background, targeted use of the complementary knowledge and professional competences of the co-operative research teams, a competitive knowledge centre can be developed, which will also be a significant player in the European research space, ensuring outstanding results at international level and realistic H2020 application potential. Sub-programme 1: Micro- and nanodiagnostics (leader: András Guttman) The sub-project includes three different topics — Molecular Diagnostics, Biosensorics, Nanotoxicity — which complement each other synergistically, taking advantage of the alternative methodological approach of different disciplines. The objective of molecular diagnostics is to develop new integrated molecular diagnostic technologies that are suitable for detailed, high-resolution glycomic profiling of samples of human origin, especially the separation and identification of glycoproteins for diagnostic and therapeutic purposes from a limited number of cells. These glycoproteins may be specific biomarkers for diseases. A number of clinically relevant studies cannot be performed using currently available analytical procedures if only very small amounts of samples are available. The proposed research theme includes the development, optimisation and integration of samples collection for molecular diagnostic purposes, sample preparation, analysis based on highly sensitive microfluidics, combined mass spectrometry and bioinformatics. The key to microfluidic sample preparation is the use of nanoparticles, as in this case the targets (tumor cells, viruses, bacteria, etc.) and the systems used to test them fall within the same size range, thus ensuring intensive phase contact. The bioanalytical molecular diagnostic methods to be developed use super-paramagnetic nanobeads, the surface of which is activated by the oriented immobilisation of specific molecules capable of producing bio-affinity bonds (e.g. antibody, lectin, aptamer). Pathogenic nano bead conjugates can be retained from the sample by a sufficiently strong focused magnetic field, so they can be separated with high efficiency from other blood constituents that make detection difficult. After microfluidic capture of glycoproteins obtained from pathogens, the designation of enzymaticly split glycanes with the fluorphor group is followed by high-efficiency capillary electrophoresis separation and direct attached ultrasensitive and high-resolution mass spectrometry analysis. Complete sample preparation is carried out in integrated microfluidic units. The Biosensorics Partema aims to develop sensor surfaces that can be used in electrochemical capacitive sensors. The sensors to be formed can be integrated into microfluid devices, thus providing direct information from biologically relevant systems. Sensing surfaces may consist of mixed monomolecular layers consisting of amphiphilic molecules or polymer/nanoparticle composite thin layers; composition of layers me