The government’s declared goal is to develop sport into a strategic sector, focusing on infrastructure and human resource expansion. The aim of the planned excellence workshop is to improve the latter’s quality, where we will research the methods of legal increase in sport performance, using the most modern fine analytical, blood-biochemical and molecular process analysis toolkit. The Hungarian Olympic Committee, which intends to organise the 2024 Olympics, also encourages the practical use of the results. By expanding the existing equipment pool and the research community at PTE, and with the help of basic scientific and applied research based on the available large sample athletes’ dataset, we will provide useful results for practitioners, which can be realised later in innovative tool developments. Diagnostics and performance enhancements supported by molecular foundations are also sporadically sporadic in Hungary and other major sports powers, taking this into account this proposal is a unique opportunity. In line with the objective of the National Intelligent Specialisation Strategy, the primary and long-term goal of this application is to strengthen the R & D capacity of our existing knowledge bases in order to produce internationally listed, high-quality research results. The Centre of Excellence for the purpose of the application combines the facilities of preventive and curative medical, sport science and performance diagnostics, molecular impact analysis and fine analytics research at our university. We hope that a combination of these would create a multidisciplinary research potential at the international forefront of the field of science. Monitoring of athlete performance based on molecular markers is not a new problem. The success of such attempts and the comparability of the results of the different centres is greatly limited by the differences in sampling and processing protocols and by the fact that the performance diagnostic tests underlying the process are performed under non-standardised conditions. All interventions and tests in the proposed centre of excellence would be carried out under standardised conditions, sampling, processing and storage would be based on optimised protocols. Furthermore, by aligning the perspectives of researchers with very different disciplines in the centre, it is possible to carry out scientifically valuable studies already at the design stage. To our knowledge, the centre of excellence proposed in the application would be the first research organisation with such characteristics in Hungary. In our studies we strive to use non-invasive sampling techniques, or to diagnose and monitor as small as possible. Our project supports the “Sporting Nation” concept, the effective operation of sports academies and clubs. Coordination of basic and applied research would develop research potential and performance in a self-exciting way. Sport science and sport medicine are characterised by the fact that the number of citations per average article in the field’s Q1 journals is in the range 0.8-2.8. Accordingly, research that inspires significant international interest must go beyond standard standards in terms of applied methodology, target group or topic. It must therefore be able to appear not in those specialist journals, but primarily in scientific journals of a broad scientific spectrum, physiological or medical. The proposed Centre of Excellence appears to be suitable in several respects. On the one hand, the processing of samples from tests carried out under standardised conditions with state-of-the-art methods ensures that scientific thoroughness cannot be criticised, and the studies proposed in this application, such as the full and sport-specific assessment of athletes, the determination of load physiological risk rates for free games, the identification of performance-related molecular markers, the separation of genetic potential and Exercise from each other, the issue of over-training or the examination of micro-injuries are among the most researched topics in the field. At the same time, a multidisciplinary approach can make such research suitable for achieving high standards and of interest to the wider scientific public. In the course of our project, we examine the anthropometric, load physiological, biomechanical and molecular parameters of individual and team sports as a function of performance. Our methodological studies can be divided into three groups. 1. Performance Physiology: With proprietary software, we monitor performance indicators using dynamometric, electromyographic, electro- and transcranial magnetic stimulation techniques, in addition to monitoring cardiovascular parameters. 2. Finomaanalysis: Fine-analytical analysis of the blood and saliva samples obtained during the load with HPLC and the mass spectrome of LCMS-Q-tof