A) In the present R & D project, we integrate genomic cutting-edge technologies into research programs at the research workshop groups. The aim of the project is to ensure that our research programmes are at the international forefront of their field of expertise, and that thanks to the various applications and technological developments implemented in the project, we create a sustainable centre of R & D & I excellence. The present research programme is based on the R & D infrastructure development of the winning GINOP-2.3.3.-15 “Development of the R & D infrastructure of the plant science knowledge and service centre based on cutting-edge technologies at the University of Pannonia”. In this tender, among other things, a Titan MC microarray device using Axiom technology is purchased, which has been marketed 5 years ago and is available only at our institution in the Central European region of 18 countries. The Axiom system offers an exponentially wider study palette than any other microarray system, and the repeatability of experimental results is practically 100 %. In addition, the system is sufficiently flexible, so it is also suitable for array development based on its own research. Another QuantStudio 7 qPCR device (LifeTechnologies) is purchased in our infrastructure development tender, which is another key element of the studies carried out in this project. The third key element is Illumina’s next generation sequencing technology, designed for small sample testing, but also for short sequencing of the largest genomes, and therefore extremely flexible system, NextSeq 500 System, which we wish to acquire in the present project. The combined availability of these three high-tech genomics technology provides complex, flexible and very wide range of test options, as follows: 1. In a single reaction, we can test hundreds of samples with microarray technology. We get tens of thousands of genomes per sample. Therefore, this method is used for the simultaneous rapid detection of variations of a large number of known genes. The specific cost of a sample is a fraction of the total genomic or transcriptive sequencing. 2. In each research programme, the most favourable and unfavourable genotypes/samples are sequenced using NextSeq. Here, in the first step, a reference genome is created from the most valuable genotype of the test, then transcriptom sequencing on the same genotype is performed and the working genes are localised in the reference genome. In the same way, we also prepare the transcript of the most unfavourable genotype and characterise the differences between the two genotypes. Of course, the reference genomes available in public databases will be of great help in compiling reference genomes made of our own valuable genotypes. 3. The expressions of genes and gene variants that control the property as defined in previous studies are analysed using qPCR for expression changes in various developmental stages, tissues and environmental effects. With the help of these tests, the genetic background of complex properties such as biotic stress responses, nitrogen utilisation capacity or drought tolerance can be interpreted. Among the programs of the research workshop groups, potato resistance breeding, vine rootstock and nobility breeding, paprika breeding, arable long-term experiments and pig farming have a history of more than 50 years and have great international recognition in their field of expertise. They have a history of decades and, thanks to international cooperation in recent years, our research on poultry feed is developing dynamically. New research is our comprehensive genomic and technological development programme on honeybees, in which we also work with partners from Hungary and abroad. In addition, we have a pear, potato and grape gene bank containing valuable gene resources, especially resistance genes, which are an important step in making our breeding projects more effective. B) R & D tasks To be completed The Titan MC platform (Axiom microarray technology) appeared 5 years ago, while NextSeq and QuantStudio 7 qPCR were released 2-3 years ago. The novelty of the research is the construction of these 3 high-tech technologies on each other: microarray technology describes the individual test materials in large sample studies, then comparing the DNA profiles obtained by NextSeq the most favourable and unfavourable genotypes for the properties tested, and the expression profile of genes that determine the property control (development stage, environmental impact, tissue type, etc.) is examined by qPCR. Based on the results obtained, new, specific, i.e. the owner