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project info
Start date: 1 June 2016
End date: 28 February 2022
funding
Fund: European Regional Development Fund (ERDF)
Total budget: 2 551 013,90 €
EU contribution: 2 551 013,90 € (100%)
programme
Programming period: 2014-2021
Managing authority: Nemzetgazdasági Minisztérium Gazdaságfejlesztési Programokért Felelős Helyettes Államtitkárság
beneficiary

Development of the R & D infrastructure of the Plant Science Knowledge and Service Centre based on state-of-the-art technologies at the University of Pannonia

A. Objectives of the project in the agro-genomic field: — Development of the R & D & I+Sz equipment park and — implementation of a greenhouse investment in order to cultivate normal experimental plants and to cultivate transgenic plants in safe, compliant conditions. New assets to be procured: — Gene Titan MC System (Affymetrix) for Axiom microarry testing in the following areas: The Axiom microarray system currently offered for 52 species of plants and animals is used to identify genes, alleles, SNPs (single nucleotide polymorphisms), genotyping, gene expression tests, genotype-environmental interaction studies, and marker-based selection in breeding programmes, analysis of complex genomes among many other approaches. — Hybridisation chamber: to perform hybridisations in microarray technology — QuantStudio 7Flex real-time PCR system: SNP tests, allele detection, complex gene expression tests — Vacuum DNA/RNA isolator + pipette tip washer: our existing pipette robot (Starlet, Hamilton) to expand function, increase efficiency, marker-based selection services — Computer and peripheral devices: for bioinformatics analyses without supercomputer — Bioanalyser (Agilent 2100): for accurate measurement of molecular purity and concentration — Mikrotom: engraving for gene expression tests — Fluorescent microscope with digital camera: gene expression test, cell count — Laboratory general tools and equipment: deep freezer, autoclave, micropipette set, cooling-heating block, orbital shaker, electrophoresis apparatus, gel documentation system, vacuum centrifuge, plate-vortex Immaterial device: — Bioinformatics analysis software (CLC Genomics) New building investment: Greenhouse: genetically modified plants necessary for functional testing of genes with individually air-conditioned, pollen-proof boxes (e.g.: hypoallergenic ragweeds) and normal experimental plants. Objectives of the project in the field of potato research: Construction of a containerised, refrigerated potato storage facility with the associated modern potato storage machine system. The project envisages the acquisition of a modern containerised, refrigerated potato storage and storage equipment system for the storage physiological research of the Centre, the storage, processing, characterisation and evaluation of breeding lines in the field of breeding and agrotechnical research, as well as for the loading and processing of seed tuber lots resulting from the maintenance and propagation of seed tubers of its own varieties (receptor, grading machine, bag scales, forklift). In addition to research tasks, domestic producers and regular foreign deliveries also require a research institute operating within a state framework to ensure the best available quality at the highest possible technical standards. Greenhouse: The Potato Research Centre intends to maintain the parental lines included in the crossing program on the 1 greenhouse surface designed for it, and to perform the planned sexual crossing work on them. At present, most of this work takes place on arable land, where the success of crossings is rendered unpredictable by weather conditions. Furthermore, the greenhouse with controlled temperature and light conditions will be very suitable for virological and phytophoto resistance tests and for performing the trusting phenotyping work required for molecular biological experiments. During the implementation of the project we implement almost all disciplines in the field of horticultural research. — The collection, mainly sorting and maintenance under controlled conditions of indigenous valerian populations in Hungary, in order to provide a basic pharmaceutical material for climatic tests. — Development of propagation and cultivation techniques related to foliage evergreen ornamental shrubs, involving several species in experiments and testing them in climate chambers. — Progeny testing of pepper breeding lines under controlled conditions, carrying out tests for grafting vegetables. — In vitro maintenance, rooting and microinoculation of Hungarian pears. — Technological development of grape propagating material production. — Vine rootstock — noble interaction test under different climatic conditions. Dr. László Kocsis, professor, is responsible for the implementation of the tasks in the field of horticultural research and development. The greenhouse to be created under the project would significantly improve the infrastructure of plant protection research and would greatly help research at the Plant Protection Institute. Our Institute’s staff carry out research work in five disciplines: herbology, plant disease, entomology, toxicology and microbiology. Our Institute has a well-equipped plant protection laboratory where we can perform screening tests,

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