A) Presentation of the tasks to be implemented during the project is one of the most dynamically developing sciences of today, cellular imaging is now suitable for high-speed (150 µs exposure time), super-resolation (XY/Z resolution 20/50 nm) testing of live cells. Comparing the results of molecular dynamics and superresolative morphological studies can revolutionise the vision of cells in all areas of life sciences, from molecular biology to neuroscience. To achieve this revolutionary and internationally unique research direction, the Consortium intends to create a unique set of tools to track rapid subcellular changes in living tissue, as well as to implement the dynamic analysis of super-resolation morphology and molecules. The tools for specific purposes are also well suited to the microscopic developments of the consortium members. Tasks to be implemented in PTE CoVE: Currently, the CoVE has a “structured illumination” microscope (SIM) for superresolation tests and is being procured as a stochastic optical reconstruction microscope (STORM) for testing live cells. Using the resources of the application, STORM provides researchers with a unique development that allows not only super-resolation but also the timely tracking of individual molecules. In order to study interactions, a STED-Flim superresolation microscope is also warranted. In addition to the complete internal reflective microscope (TIRFM) in CoVE, a tool will be built that can track individual molecules in a living cell with unique speed in the world using the highly inclined laminated optical sheet (HILO) technique (3D-SMD). An in vivo 2-photon microscope is also procured for the monitoring of rapid subcellular processes in live animals. The tasks to be implemented in the SZBK are: Opens new dimensions of the exploration of anisotropic molecular structures — in a way that significantly exceeds the technical capabilities of the DP-LSM previously developed in the SZBK, by increasing lateral resolution and sensitivity — the acquisition of a new innovative process and equipment equipped with a differential polarisation condition, the re-screening concocal microscope (RCM, Re-scan Confocal Microscopy). New equipment also complements the in vivo 2-photon microscope to increase subcellular resolution. Tasks to be implemented in DE: DE acquires a time-corrected unique photon counter (TCSPC) microscope toppings, which is a top-notch instrument that fills the gap in Hungary, with which the molecules can be detected with unique molecular sensitivity at the same time as the 2-10 nm co-consistency, the bonding state, the co-mobility and the degree of aggregation. B) Presentation of the suitability of the implementation site(s). Complemented by existing imaging capacities and the expertise of the three institutions, a unique array of microscopic and in vivo imaging instruments of a wide spectrum and quality will be created in Central Europe. The developments are carried out in laboratories with significant infrastructure (PTE-SzKK, DE, SZBK) which are still operational for all partners. The PTE research groups included in the tender are working in the modern “SMART building technology” building of the CoVE, built in 2012, the infrastructure of which is excellent for the installation and maintenance of the planned instruments. In individually air-conditioned, fully-darkable rooms, it is planned to accommodate the instruments in such a way that, even in the case of further extensions, sufficient space for the use of the instruments is not disturbed. The laboratories participating in the tender have an internationally outstanding instrument park (SIM, STORM, TIRF, HILO, DP-LSM, Flim, in vivo two-photon microscopy). Internationally recognised experts in microscopy (Istvan Ábrahám, Zsuzsanna Jóes, Győző Garab, György Vereb, Csaba Varga, István Krizbai, Boldizsár Czéh, Dóra Reglődi and György Vámosi, the Hungarian coordinator of Euro-BioImaging) ensure a high level of expertise and maximum utilisation of the development. The PTE CoVE, the DE Microscopic Service Laboratory and the SZBK have 2 full-time qualified staff, and several research teams work part-time. To create the Nano-BioImaging core facility, PTE will create 2 new research jobs. The long-term replacement is provided by 3 doctoral schools and students of the Medical Imaging Laboratory Diagnostic Analytical Department at DE and PTE. The scientific metric data of the staff working in the institutes are summarised below. Ábrahám István: 49 Communications, IF:202, CIT:1953, H-index:22; György Vámosi: 60 Communications, IF:234, CIT:1783, H-index:22, patent:1; Good Zsuzsanna: 135 Communications, IF:401, CIT: 2852, H-index: 30, patent:11; Boldizsár Czéh: 58 Communications, IF:231, CIT:4198, H-index:29; Csaba Varga: 22 Communications, IF:218, CI
