City transport is facing the challenges posed by the new technologies and by the undergoing socioeconomic and demographic changes, seeking for more efficient solutions and mobility services tailored to citizens’ new travel behaviors and needs. TRIBUTE project aims at creating and testing integrated innovative tools and actions in the Adriatic-Ionian (AI) cities, by means of the creation and the implementation of eight living labs on sustainable urban mobility. These will be the mean to discuss transport policies and solutions (best practices), and to share views about the future needs following a participatory approach involving governmental institutions, businesses, researchers, and citizens (i.e. the quadruple helix). The results will converge into eight action plans and pilot actions including innovative and sustainable solutions ranging from innovative public transport and on-demand responsive services using electric vehicles, to “green” cycling routes network and integrated management systems of public transport and cars along highly congested corridors. In so doing, TRIBUTE will promote transnational cooperation between public authorities and their transport entities, allowing for the creation of a transnational strategy devoted to policymakers and planners towards new mobility agendas for sustainable, integrated, and inclusive transport system in the AI Region.