At a time when a significant number of children are the product of assisted reproduction (MAP), it is necessary to develop procedures for characterisation, selection, and conservation of gametes of the highest quality for LDCs (as for AI of productive animals). Many men produce sperm that are characterised by high levels of DNA damage, which appear to be largely induced by oxidation. The presence of DNA damage has been linked to an increased incidence of miscarriages and an increased morbidity profile in offspring, including complex neurological disorders, such as spontaneous schizophrenia or autism, and cancer. The axes developed in the project are both fundamental and applied allowing by this acquired expertise, the emergence of a structure of services and R & D dedicated to the evaluation of the sperm profile and the nuclear quality of male gametes (for species of agronomic interest as for infertile humans).