The project "Microorganisms and Biological Tissues: from equilibrium to pathology" will aim to describe the role of interactions between microorganisms (or metabolites produced by them) and biological tissues in three contexts: — a normal situation (cell growth), – a situation of intestinal pathology (inflammation of the type of Crohn’s disease), – an associated extra-intestinal pathological situation (migraine). To meet the objectives of this project, high-resolution imaging is essential. The acquisition of state-of-the-art equipment, installed on 2 Clermontese platforms, has become a must and priority. An innovative approach will be implemented by the correlation of the different modes of imaging of increasing resolution (photonic/confocal/electronic). Additional information on the structure, function, dynamics (in vivo and real-time imaging) and chemical composition of the model studied can be obtained. This structuring project involving several Joint Research Units (UMR) of the Clermontois site (GreD, Neuro-Dol, M2iSH, IMoST) and associated platforms (CICS, GReD-CLIC) aims to answer the questions that are currently key points in the field of the role of the intestinal microbiota and its metabolites on physiology and pathology.