The goal of the FlowPROSPER project is to develop advanced Flow Cytometry Protein Signature platform and service for the biomedical research and R&D sector, in the field of personalised therapies. Civilisation diseases, including cancer, cardiovascular and neurodegenerative diseases, are the major socio-economical burden for societies worldwide. Development of personalised therapies to treat civilisation diseases is a current line in the biomedical sector. Also, designing of novel methods and markers to diagnose susceptibility to treatment and effectiveness of the therapy is important. Specifically, we plan to create the advanced multicolor flow cytometry service to analyse the specific protein signatures, which can be used to predict sensitivity to personalised therapies or even monitor the response and efficiency of treatment. This panels can be designed for a broad range of biomedical proof-of-concept, Preclinical and clinical studies, in a multiple civilisation diseases.